A Guide to CRISPR-Cas Mechanism of Action: Function and Optimization
The Missing Link in Pre-Clinical Genome Editing Workflows Currently, the “gold standard” genome editing workflows only produce one data type: in-cell editing events. With this single parameter, there is just one outcome to optimize around. Discovery programs typically focus on guide RNAs (gRNA) redesign or transfection conditions to maximize sensitivity and selectivity to troubleshoot CRISPR-Cas […]
